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KMID : 1191420090080010001
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Korean Journal of Optometry and Vision Science
2009 Volume.8 No. 1 p.1 ~ p.8
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Bevacizumab Effects on Vascular Endothelial Growth Factor of Cultured Retinal Pigment Epithelial Cell
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Lee Young-Chang
Hau Kwang-Sun
Choi Gwang-Ju
Kim Dae-Hyun
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Abstract
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Purpose: To examine the effect of Bevacizumab on vascular endothelial growth factor of cultured human retinal pigment epithelial cells.
Methods: Bevacizumab was exposed on human retinal pigment epithelial cells which cultured in hypoxic environment and expression and production of VEGF were tested by Western blot. Angiogenesis was measured with a tube formation assay that uses ECV 304 cells (American Type Culture Collection) and with a migration assay that uses human dermal microvascular endothelial cells.
Results: Expression and production of VEGF tested by Western blot respectively were reduced in Bevacizumab exposed retinal pigment epithelial cells which culured in hypoxic environment (p＜0.05). Tube formation and cell migration were reduced which tested using ECV 304 cells (p＜0.05).
Conclusions: These results suggest that Bevacizumab suprpresses the secretion of VEGF by RPE and neovascularization.
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KEYWORD
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Angiogenesis, Bevacizumab, VEGF
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